• 专利附录
  • 专利说明
  • 权利要求
  • 类似技术
  • 同族专利
  • 引用文献
  • 法律状态
  • 优先权
    • 专利摘要:
    An agent for the elimination of turbidity in serum, especially in serum samples wherein photometric measurements are to be made, consisting essentially of one or more mono- or di-esters of polyethylene glycol and fatty acids of 9 to 14 carbon atoms with at most 8 ethylene oxide units per fatty acid moiety in the molecule, said esters having an HLB value of from 8 to 12.8.
    公开号:SU791267A3
    申请号:SU772532397
    申请日:1977-09-13
    公开日:1980-12-23
    发明作者:Батц Ханс-Георг；Дрегер Бригитте；Вильгельм Валефельд Аугуст；Вайманн Гюнтер；Грубер Вольфганг
    申请人:Берингер Маннхайм Гмбх (Фирма)；
    IPC主号:
    专利说明:

    The invention relates to biochemistry and can be used in medicine for the purification of preparations. A means for cleaning the sweep of photometric measurements is known, which contains an emulsifier for impurities and a solvent 1}. However, with the application of a known agent, a slight clarification of serum is achieved. The purpose of the invention is to enhance the reactivity of the agent. This goal is achieved by the fact that the agent contains one or more polyethylene glycol mono- or diesters (from 1 to 8 ethylene oxide units) and lauronic acid, as an emulsifier, and contains a substance selected from the group: alkanol (with 1–3 atoms carbon), glycol (with 1-7 carbon atoms), polyglycol (with 1-8 ethylene oxide units), polyethylene alkanol ether (with 8-16 C atoms in a molecule containing 8-14 polyethylene oxide units), and components are taken in the following ratio, wt.%: Mono- or di-ether of polyethylene glycol and lauri New acid 10-40, Solvent Else Lauric acid is used as an acid part in the agent, but pelargonic acid, capric acid, undecyl acid, tridecylic acid or mristristic acid can also be used. Especially preferred results are achieved if the proportion of esters is 10-40 wt.% And 3-4 units of ethylene oxide in the molecule. The ester component in the vehicle is not purely soluble and therefore causes further turbidity when added to the serum. However, depending on the composition of the serum, when an agent is added, the turbidity disappears within a few minutes and a completely transparent serum is obtained. But in another case, it is necessary to additionally add a solubilizing agent. Low alkanols, in particular ethanol with 1–3 atoms, glycols up to 6 C atoms, polyglycols with B units of ethylene oxide, or surface active substances, which themselves can not brighten dimly in serum, like solvating agents, are used as solubiating agents. . Polyethylene glycol ether, especially polyethylene glycol ethers of alkanols with a content of 8–16 C atoms per molecule, which contain 8–14 ethylene oxide units, are used as surfactants. Avoiding contamination is very important. The product must not contain traces of alcohol and acid. Therefore, polyethylene glycol esters which are commercially available are not suitable without further purification. During the purification, the polyglycols contained in them are also separated. Purification is carried out by extraction by shaking aqueous solutions of halogenated hydrocarbons, such as trichloro, with ethylene. Get the tool as follows. Polyethylene glycols with 3-8 units of polyethylene oxide in a molecule are reacted with boric acid in a molar ratio of 3: 1, the resulting product is heated with a fatty acid of 9-14 C atoms, then hydrolyzed by adding water, extracted with organic mixing water, solvent; the organic phase is separated; dried; treated with alumina; and then alumina and solvent are separated. Extraction of the solvent is carried out from the salt-saturated aqueous solution. Halogenated solvents, for example, chloroform, are used as an extracting agent. The reaction with boric acid is carried out at a temperature of 7 ° C for 7 hours under vacuum. The ester of boric acid esters obtained from fatty acid esters of polyethylene glycol are suitable for the agent, since they are of high purity, however, commercial esters can also be used to prepare the agent. In this case, low molecular weight esters are added, i.e. esters with 3-4 units of ethylene oxide in the molecule, in the amount of 5-20 wt.%. The agent may also contain a buffer solution and other inert substances to the serum components and used in the assay systems. The agent is added in such quantities as to achieve the desired clarification effect. These amounts are 0.1-5%, depending on the composition of the serum to be clarified and other reagents added to the serum. However, you can use a smaller amount of funds. If large amounts of the agent are required, a low alkanol with 1-3 C atoms or glycol with 1-7 C atoms or polyglycol with 8 units of ethylene oxide in the molecule as a solubilizing agent is added. This takes into account the sensitivity of the components in solution to alcohols. If there are enzymes sensitive to alcohols, then a surface active substance is used as a solubilizing agent. If, for example, glucose is determined by the method of glucose oxidase, then 0.4% of the ester and 0.1-0.4% of the solubilizing agent are added, if using catalase and aldehyde dehydrogenaene are determined on a cloudy serum uric acid sample, then means of an appropriate 3% by weight ester. If a surfactant is used as a solubilizing agent, then it is added in a minimal amount. The agent eliminates even significant serum turbidity. In many cases, the addition of an agent allows optical measurements to be performed on sera with unremoved protein. Therefore, the agent is suitable for both substrate and enzyme determinations. Example 1. Preparation of an agent. 2 mol of polyethylene glycol with 3-3 units of ethylene oxide is heated with 0.66 mol of boric acid for 2 hours to a vacuum obtained with a water jet pump. The composition is then kept at this temperature for another 6 hours. After cooling, 0.2 mol of lauric acid and 2 g of p-toluene-sulfonic acid are added. Then it is heated for 2 hours before and for another 2 hours soaking at this temperature. Then, the same volume of water is added for hydrolysis and stirring is carried out at 1 hour. The solution is then saturated with sodium chloride and extracted three times with shaking with ethyl acetate. The combined ether phases are dried over sodium sulfate and then concentrated to approximately 100 ml, then the solution is shaken with 50 g of basic alumina. The thin layer chromatogram is used to check the absence of fatty acids. If the fatty acids still remain, repeat the treatment with alumina. The alumina is then filtered off and the solvent is removed using a vacuum generated by an oil pump.
    权利要求:
    Claims (1)
    [1]
    Claim
    A means for cleaning serum during photometric measurements, containing an emulsifier for impurities and a solvent, characterized in that, in order to enhance the activity of the agent, it contains one or more mono-or diesters of polyethylene glycol (from 1 to 8 ethylene oxide units) and lauric acid as an emulsifier acid, and as a solvent it contains a substance selected from the group: alkanol (with 1-3 carbon atoms), glycol (with 1-7 carbon atoms); polyglycol (with 1-8 units of ethylene oxide), ether of polyethylene alkanol (with 8-16 C atoms in a molecule containing 8-14 units of polyethylene oxide), and the components are taken in the following ratio (wt.%): monoyl - polyethylene glycol diester and lauric acid 10-40, solvent - the rest.
    类似技术:
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    同族专利:
    公开号 | 公开日
    DK144668B|1982-05-03|
    JPS5715340B2|1982-03-30|
    GB1542982A|1979-03-28|
    ZA775815B|1978-09-27|
    DE2724757C2|1979-08-23|
    ATA612577A|1979-06-15|
    HU176755B|1981-05-28|
    IE45675B1|1982-10-20|
    CH635437A5|1983-03-31|
    DK144668C|1982-10-04|
    FR2393291B1|1980-08-01|
    AR212779A1|1978-09-29|
    US4184848A|1980-01-22|
    FI62421B|1982-08-31|
    AU2916277A|1978-07-27|
    NL7800010A|1978-12-05|
    CA1121381A|1982-04-06|
    FI62421C|1982-12-10|
    IL52986D0|1977-11-30|
    AT354641B|1979-01-25|
    DD131970A5|1978-08-09|
    DK422677A|1978-12-02|
    DE2724757B1|1978-12-21|
    SE428474B|1983-07-04|
    IL52986A|1980-03-31|
    IT1086033B|1985-05-28|
    JPS53149390A|1978-12-26|
    SE7710630L|1978-12-02|
    NL172486C|1983-09-01|
    BE858883A|1978-03-20|
    FR2393291A1|1978-12-29|
    FI772812A|1978-12-02|
    IE45675L|1978-12-01|
    引用文献:
    公开号 | 申请日 | 公开日 | 申请人 | 专利标题
    US3770631A|1971-06-29|1973-11-06|Baxter Laboratories Inc|Clarification of blood serum and plasma|
    US3853465A|1972-06-09|1974-12-10|Technicon Instr|Turbidity reduction in serum and plasma samples using polyoxyethylated lauric acid compounds|
    US3847482A|1972-07-10|1974-11-12|Bio Data Corp|Apparatus for detecting a change in turbidity of a solution|
    US3958939A|1975-01-08|1976-05-25|Coulter Electronics, Inc.|Method for clarification of lipemic serum|
    US4011045A|1975-02-14|1977-03-08|Bonderman Dean P|Turbidity reduction in triglyceride standards|DE2816229C2|1978-04-14|1983-11-10|Boehringer Mannheim Gmbh, 6800 Mannheim|Methods and means for removing opacities|
    DE2829531A1|1978-07-05|1980-01-24|Heuck Claus Christian Dr Rer N|METHOD FOR QUANTITATIVE DETERMINATION OF A SERUM PROTEIN IN BLUE SERUM AND PLASMA SAMPLES|
    DE2839433A1|1978-09-11|1980-03-20|Merck Patent Gmbh|STANDARD AQUEOUS LIPID SOLUTION AND METHOD FOR THEIR PRODUCTION|
    AT390T|1978-11-01|1981-11-15|Contraves Ag|METHOD FOR REDUCING THE INTERFERENCE OF A PHOTOMETRIC MEASUREMENT BY LIGHT DISTRIBUTION IN A SUSPENSION TO BE MEASURED AND REAGENT FOR CARRYING OUT THE METHOD.|
    US4330622A|1979-09-12|1982-05-18|Becton Dickinson & Co.|Elimination of non-microbial turbidity in culture media|
    US4319882A|1980-02-21|1982-03-16|Yash Sharma|Method for detecting immunological agglutination and biochemical agent therefor|
    US4411795A|1980-03-10|1983-10-25|Baxter Travenol Laboratories, Inc.|Particle adsorption|
    DE3021457A1|1980-06-06|1982-01-07|Boehringer Mannheim Gmbh, 6800 Mannheim|METHOD AND MEANS FOR SOLVING CHYLOMICRON IN AQUEOUS MEDIUM|
    CA1163908A|1980-10-01|1984-03-20|Shyun-Long Yun|Method for eliminating turbidity in a biologicalfluid and reagent therefor|
    DE3107060A1|1981-02-25|1982-09-09|Boehringer Mannheim Gmbh, 6800 Mannheim|CONTROL OR CALIBRATION SERUM AND METHOD FOR THE PRODUCTION THEREOF|
    US4506018A|1982-12-30|1985-03-19|Becton, Dickinson And Company|Blood diluent|
    JPS59162454A|1983-03-08|1984-09-13|Kainosu:Kk|Removal of turbidity in humor|
    DE3323949A1|1983-07-02|1985-01-03|Boehringer Mannheim Gmbh, 6800 Mannheim|METHOD AND MEANS FOR RAPIDLY AND COMPLETELY ELIMINATING A TURBIDITY IN A BIOLOGICAL LIQUID|
    JPS6247335U|1985-09-09|1987-03-24|
    FR2599149B1|1986-05-21|1988-08-26|Univ Nancy|REAGENT FOR TRANSPARIZING BIOLOGICAL MEDIA AND ITS ANALYTICAL APPLICATIONS.|
    DE3920364C2|1989-06-22|1993-04-29|Technicon Gmbh, 6368 Bad Vilbel, De|
    JPH0395836U|1990-01-19|1991-09-30|
    DE19850074A1|1998-10-30|2000-05-04|Dade Behring Marburg Gmbh|Stabilization of biological liquids by adding sterol esters|
    EP1233269B1|2001-02-19|2003-11-19|Olympus Diagnostica GmbH|Agent for the removal of turbidity in biological samples|
    法律状态:
    优先权:
    申请号 | 申请日 | 专利标题
    DE2724757A|DE2724757C2|1977-06-01|1977-06-01|Means for removing cloudiness in serum and process for its preparation|
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